As part of an assessment of habitat use and movements of odontocetes being undertaken in the western half of the main Hawaiian Islands (see Baird et al. 2011, 2012), field operations funded by Commander, U.S. Navy Pacific Fleet were undertaken off of Kaua‘i in January 2012. There were two primary goals of these operations: 1) providing species verification for acoustic detections by the Marine Mammal Monitoring on Navy Ranges (M3R) project on the Pacific Missile Range Facility (PMRF) hydrophone range (see Moretti et al. 2012); and 2) deploying satellite tags prior to a Submarine Commanders Course (SCC) training event to be undertaken off Kaua‘i in February 2012.

Movements and Spatial Use of Satellite‐tagged Odontocetes in the Western Main Hawaiian Islands: Results of Fieldwork Undertaken off O‘ahu in October 2010 and Kaua‘i in February 2011

Here we combine results of field efforts funded through the U.S. Navy’s Living Marine Resources Program (through a three-year grant from the Naval Postgraduate School) as well as additional field time in the study area in 2011 and 2012 funded through the Pacific Islands Fisheries Science Center and Commander, Pacific Fleet. We also utilize results from research efforts in the eastern main Hawaiian Islands, off Lāna‘i, Maui, and Hawai‘i Island, to help interpret results from photo-identification and satellite tagging.

As part of a long-term U.S. Navy-funded marine mammal monitoring program, in February 2020 a combination of vesselbased field effort and passive acoustic monitoring was carried out on and around the Pacific Missile Range Facility (PMRF) off Kaua‘i prior to a Submarine Command Course scheduled for mid-February 2020. The purpose of the monitoring effort was to assess the spatial movement patterns and habitat use of cetaceans that are exposed to midfrequency active sonar and how those patterns influence exposure and potentially responses. Results from this effort were compared with previous Cascadia Research Collective (CRC) survey effort and photo-identification and tag data from Kaua‘i, based on surveys in 11 different years since 2003.

As part of the long-term United States (U.S.) Navy-funded Marine Species Monitoring Program, from 11–19 February 2024, Cascadia Research Collective (CRC) carried out a vessel-based field effort in conjunction with passive acoustic monitoring undertaken by the U.S. Navy (Navy) scientists on and around the underwater hydrophone ranges of the Pacific Missile Range Facility (PMRF). The effort was timed to occur immediately prior to the start of a Submarine Command Course (SCC) to allow for collection of movement and dive data that could be used to examine exposure and response of cetaceans to Navy mid-frequency active sonar (MFAS; see Henderson et al. 2021). Eight days of field effort were funded by the Navy, and one day of field work was funded by National Oceanic and Atmospheric Administration (NOAA) Fisheries. Wildlife Computers SPLASH10-F satellite tags were funded by the Navy for deployment on Navypriority species, and additional tags were provided by Wildlife Computers or CRC for deployment on other species (e.g., humpback whales [Megaptera novaeangliae]). Tags were programmed to collect dive behavior and Fastloc®-Global Positioning System (GPS) data from the time deployed until three and a half days after the end of the SCC and continue to transmit the dive and Fastloc®-GPS data for an additional six days. Starting on either May 2nd or May 3rd, the tags were duty cycled to transmit only every second day, to provide longer-term tracks of individual movements. During the period that tags were collecting dive and Fastloc®-GPS data, tags were programmed to try to obtain Fastloc®-GPS locations only for the 12 hours of the day when there were no or limited satellite overpasses. This programming change from previous Kauaʻi field efforts was taken to minimize temporal gaps in location data during the period that overlapped with and immediately followed the SCC. Field efforts were undertaken out of Kikiaola small boat harbor when conditions allowed for working on or near PMRF, or out of Port Allen when conditions on or near PMRF were not workable.

Evidence from strandings and anecdotal reports indicate that a number of odontocete species interact with near-shore fisheries in Hawai'i. In the absence of observer programs in these fisheries, we evaluated mouthline injuries from known resident populations of false killer whales and pygmy killer whales, to assess the viability of this method to document injuries associated with hook and line fishery interactions. All individuals with mouthlines visible were selected from photo-ID catalogs and scored for presence of mouthline injuries consistent with fisheries interactions. Ninety-seven false killer whales and 47 pygmy killer whales had ≥50% of the mouthline visible using fair to excellent quality photos, with a mean of 67% and 59% mouthline visible, respectively. Preliminary analysis suggests that main Hawaiian Islands insular false killer whales have high rates of mouthline injuries- 20% of adult and sub-adult individuals with ≥50% mouthline visible have injuries consistent with fisheries interactions, supporting studies using dorsal fin injuries that indicate individuals from this population regularly interact with fisheries. Pygmy killer whales also appear to interact with fisheries at high rates. Of adult and sub-adult individuals off Hawai'i and O'ahu with ≥50% of the mouthline visible, 31% have injuries consistent with fisheries interactions. Since pygmy killer whales feed primarily at night, and there are few reports of them depredating lines, mouthline injury analysis provides new insight into fisheries interactions for this species. Scars on pygmy killer whales heal white and are easier to detect than healed injuries on false killer whales, thus a greater proportion of individuals with such injuries may be detected for pygmy killer whales. With both species, the proportion of mouthline visible increased the likelihood of mouthline injuries being detected. Injury rates are negatively biased, since those individuals scored as having no mouthline injury may not have had their entire mouthline visible. Further efforts will aim to identify injury rates in short-finned pilot whales and rough-toothed dolphins. An examination of differences in injury rates between age classes, and multi-species comparisons will also be undertaken to better understand fisheries interactions.

The Hawaiian Islands Cetacean and Ecosystem Assessment Survey (HICEAS) is a recurring ship-based, line-transect survey designed to estimate cetacean abundance in the entirety of the Hawaiian EEZ.

The first cetacean circovirus, beaked whale circovirus (BWCV), was recently reported in a Longman’s beaked whale (Indopacetus pacificus) stranded in Hawai‘i and represents an emergent disease with unknown population impacts. In other species, circovirus infection may cause mortality or opportunistic co-infection by other pathogens. We report on a targeted surveillance of stranded cetaceans in the Pacific basin, including archived beaked whale species, strandings where pathological findings suggested disease presence, mass stranded animals, and additional individuals to represent a broad range of Hawaiian cetacean species. Archived tissues primarily from the brain, kidney, liver, lung, spleen, and lymph nodes of individuals stranded between 2000 and 2020 (n=30) were tested by PCR for the presence of BWCV.

This report presents a geographic information system (GIS)-based overview of the sighting records obtained during marine species monitoring effort (a total of 12 U.S. Pacific Fleet-funded surveys) to date in the MIRC. Also included are incidental marine mammal and sea turtle sightings made since 1965 on non-dedicated surveys in the region.

The first phase to integrate the historical sum of these U.S. Navy-sponsored visual survey efforts for marine species monitoring in the HRC was prepared by Uyeyama (2011). This report represents the second phase of that effort and presents an overview of monitoring survey effort to date in the HRC. Available data from visual surveys performed by both contractor-led efforts and U.S. Navy in-house surveys was compiled and processed in order to: 1. Create a geographic information system (GIS)-based summation of all survey tracks that can be easily visualized. 2. Construct a geo-referenced database of sightings that can be queried by species, observer platform, Beaufort Sea State (BSS), season, or other sighting/survey variables. 3. Conduct an enhanced analysis of sighting success with respect to survey platform, field protocol, and environmental conditions. 4. Inform and adjust data collection procedures on future surveys as needed to reflect lessons learned from sighting data compilation efforts presented here. 5. Provide information to enhance informed management of marine protected species in the HRC and support the adaptive management process that is part of the ICM

This report presents the results of marine mammal monitoring for the Koa Kai 11‐1 training event, and represents the continued monitoring efforts under the U.S. Navy’s Marine Species Monitoring Program. The objectives of the vessel‐based monitoring effort for Koa Kai 11‐1 consisted of the following:  Perform focal behavioral follows of marine mammals during the course of a training event involving ASW. Assess the occurrence, distribution, and behavior of target species (marine mammals and sea turtles) using a vessel‐based line transect survey within the HRC in waters within or adjacent to the area where ships were training. Locate individual false killer whales (Pseudorca crassidens), pygmy killer whales (Feresa attenuata), and short‐finned pilot whales (Globicephala macrorhynchus) tagged by Dr. Robin Baird with Cascadia Research. Investigate the occurrence, distribution, and behavior of target species (marine mammals and sea turtles) using vessel‐based line transect survey in waters within or adjacent to the area where Navy ships were training

In order to support the U.S. Navy in meeting regulatory requirements for monitoring established under the Final Rules and to provide a mechanism to assist with coordination of program objectives under the ICMP, this survey was conducted to confirm the Marine Mammal Monitoring on Navy Ranges (M3R) acoustic detections and marine mammal satellite tagging was prior to a training event, the Submarine Commander’s Course exercise, which would be conducted in the same area during February 2012.This report presents the methods and preliminary results of a vessel-based visual and acoustic survey effort that was conducted in conjunction with a tagging and species-validation project

The overall objective of the ICMP is to assimilate relevant data collected across U.S. Navy range complexes in order to answer questions pertaining to the impact of MFAS and explosives on marine mammals and sea turtles.

This report summarizes work conducted in FY2009-FY2010 with Navy support to characterize marine mammal sounds related to passive acoustic monitoring in the Hawaii Range Complex (HRC). Existing acoustic data from the Hawaii Range Complex area were analyzed to provide better descriptions of acoustic signals by species.

The Pacific Islands Fisheries Science Center’s (PIFSC) Cetacean Research Program (CRP) conducted surveys for cetaceans in the waters surrounding Guam and the Commonwealth of the Northern Mariana Islands (CNMI) (Figure 1) in an effort to further develop a record of cetacean occurrence in the region and to gather photos and biopsy samples for population studies. This project was carried out in partnership with the U.S. Navy Commander, Pacific Fleet by a team of six primary personnel (Table 1).

e Pacific Islands Fisheries Science Center’s (PIFSC) Cetacean Research Program (CRP) conducted surveys for cetaceans in the waters surrounding Guam and the Commonwealth of the Northern Mariana Islands (CNMI) in an effort to further develop a record of cetacean occurrence in the region.1 This was the fourth year conducting cetacean surveys aboard small boats (7.6 – 12.2 m) around the southern Mariana Islands of Guam, Rota, Saipan, Tinian, and Aguijan (Fig. 1). This project was carried out in partnership with the Commander, U.S. Pacific Fleet by a team of four primary personnel (Table 1). The collected data are being used to reveal the occurrence and distribution, stock structure, and movements of cetaceans within the study area.

an effort to further develop a record of cetacean occurrence in the region, as well as to gather photos and biopsy samples and deploy satellite tags for population studies, the Pacific Islands Fisheries Science Center's (PIFSC) Cetacean Research Program (CRP) conducted surveys for cetaceans in the waters surrounding Guam and the CNМІ (Figure 1) during 2010-2014. This research was carried out in partnership with the U.S. Navy and is expected to continue through 2015. Detailed reports for each survey year were submitted to the Navy (Oleson and Hill 2010, Ligon et al. 2011, Hill et al. 2012, Hill et al. 2013).

The Pacific Islands Fisheries Science Center’s (PIFSC) Cetacean Research Program (CRP) has been conducting visual surveys and long-term acoustic monitoring for cetaceans in the waters surrounding Guam and the Commonwealth of the Northern Mariana Islands (CNMI) as part of an ongoing effort to develop a record of cetacean occurrence in the region. Visual surveys have been conducted aboard small boats (7.6-12.2 m) since 2010 off the southernmost islands of the Mariana Archipelago (Guam, Rota, Saipan, Tinian, and Aguijan). These surveys include the collection of photographs for individual identification, tissue samples for genetic analysis of population structure, and the deployment of satellite tags for assessment of individual movements throughout the broader region.

The PIFSC CRP has conducted research on cetaceans in the Mariana Archipelago since 2010. A cooperative effort with funding from U.S. Navy Pacific Fleet and PIFSC has included summer and winter small-boat surveys off the southernmost islands (Saipan, Tinian, Aguijan, Rota, and Guam), development of photo-ID catalogs, analysis of collected tissue samples and satellite telemetry tag data, and shipboard visual and PAM surveys in portions of the EEZ in 2010 and 2015. PIFSC has maintained long-term PAM on moored recorders off Saipan and Tinian (since 2010) and off Pagan (since 2015) as part of the Pacific Islands Passive Acoustic Network (PIPAN). The goal of these efforts is to collect the data necessary to conduct population assessments for cetaceans within the Mariana Archipelago, including the determination of their occurrence, population structure and abundance, movements, distribution, and habitat use.

Goals: Understand species occurrence and distribution. Determine the population structure and abundance of the those species. Study the movements and habitat use of individuals and populations. Methods: Small boat-based and ship-based surveys. Passive acoustic recordings (HARPs & towed array). Photo-ID, biopsy sampling, and satellite tagging.

In this assessment we incorporated published and unpublished information to delineate and score Biologically Important Areas (BIAs) for cetaceans in the Hawaiʻi region following standardized criteria. Twenty-six cetacean species have been documented in Hawaiʻi. Eleven odontocete species have distinct small populations resident to one or more island areas: rough-toothed dolphins, pantropical spotted dolphins, common bottlenose dolphins, spinner dolphins, short-finned pilot whales, false killer whales, pygmy killer whales, melon-headed whales, Blainville’s beaked whales, Cuvier’s beaked whales, and dwarf sperm whales.

Aerial surveys of marine mammals were performed as part of the Marine Mammal Monitoring on Navy Undersea Acoustic Ranges (3MR) program. Surveys were performed in three regions: a) Pacific Missile Range Facility (PMRF), Barking Sands, Kauai (BSURE and BARSTUR ranges; Jul 12-Nov 17, 2002); b) Bahamas (Northwest Providence Channel and AUTEC range; Jan 4-12, 2003); and c) main Hawaiian Islands (Feb 21–Apr 5, 2003). The mission was to identify species and record positions and composition of all marine mammal pods sighted. These data were made available to co-investigators (Martin and Moretti) of the 3MR program in order to correlate the visual positions with acoustic localizations using Navy assets. All surveys were flown in a twin-engine Partenavia aircraft at 100 knots airspeed and 244 m altitude. Four personnel participated on each flight including one observer on each side of the aircraft, data recorder and pilot. Methods used were consistent with those of modern distance sampling theory.

With funding from the Living Marine Resources (LMR) Program, the Marine Mammal Monitoring on Navy Ranges (M3R) passive acoustic monitoring system has been installed at the Pacific Missile Range Facility (PMRF). The overall goal of the program is to provide tools and methodologies for long-term monitoring of marine mammal populations. The program is laying the groundwork needed to adapt combined passive acoustic, tagging, and visual monitoring methods for animals and conditions at PMRF. For sonar-sensitive Blainville’s beaked whales (Mesoplodon densirostris; Md), the short- and long-term distribution, density, and habitat can be measured provided basic behavioral data such as group size and dive rates at PMRF are known.

This report covers two separate activities carried out by the Cetacean Research Program at the Pacific Islands Fisheries Science Center from February to June 2009. A cetacean assessment survey of the main Hawaiian Islands was conducted by NMFS in February 2009. The survey was designed to conform to the 2002 NMFS HICEAS survey (Hawaiian Islands Cetacean Ecosystem Assessment Survey) so that abundance and distribution estimates would be comparable.

Numerous marine mammal populations worldwide are declining because of increased anthropogenic and natural threats, including infectious disease. Brucella ceti, morbillivirus, and herpesvirus have been detected from stranded cetaceans and been recognized as pathogens that may result in considerable cetacean morbidity and mortality. Beaked whale circovirus has been reported in multiple cetacean species in Hawaii, USA, and the western Pacific Ocean, but the pathologic relevance in these species remains uncertain. We screened for the presence of Brucella in 66 cetaceans of 17 species that stranded in Hawaii 2000-24 by targeting the insertion sequence 711 partial sequence using PCR.

The goal of the aerial survey was “to monitor and report the presence/absence, distribution/re-distribution, reaction/no reaction, injury, and/or mortality of MM/ST during exposure to SCC OPS Navy training event”. This involved monitoring and reporting, in as detailed fashion as possible, the surface behavior of MM/ST. In particular, we were to monitor for any changes in the near-surface behavior, orientation, occurrence, and location of animals relative to the USS Lake Erie’s activities using a systematic search and focal follow method. This included monitoring for any potentially dead, injured, or distressed animals and “any animals found to be exhibiting behaviors or associations deemed unusual”

The University of Hawaii Health and Stranding Lab located at Marine Corps Base Hawaii (MCBH) is the only entity in the Pacific Islands region that responds to strandings, conducts necropsy and cause of death investigations, archives tissues and performs research to identify and evaluate threats to Pacific Island cetaceans. This project focuses on increasing our understanding of infectious diseases in the Pacific Island region by investigating the emerging diseases caused by circovirus, morbillivirus, herpesvirus and toxoplasmosis infections in cetaceans.

The University of Hawai‘i Health and Stranding Lab (HSL), located in Kaneohe Bay, is the only entity in the Pacific Islands region that responds to strandings, conducts necropsy and cause of death investigations, archives tissues and performs research to identify and evaluate threats to Pacific Island cetaceans. The purpose of this project has three objectives: 1) To conduct comprehensive stranding investigations for high priority species through increased capacity for in-house diagnostics; 2) To analyze archived tissues for the presence of two pathogens (circovirus and morbillivirus) and to examine the detectability of Toxoplasma positive animals over time and 3) To conduct analyses of historical stranding patterns and causes of mortality that incorporate quantitative estimates of stranding date and advanced diagnostic information.

The University of Hawaii Health and Stranding Lab located at Marine Corps Base Hawaii (MCBH) is the only entity in the Pacific Islands region that responds to strandings, conducts necropsy and cause of death investigations, archives tissues and performs research to identify and evaluate threats to Pacific Island cetaceans (dolphins, whales, and porpoises).

We report significant pathological findings from 250 stranding investigations of 20 cetacean species in the Pacific Islands region between 2006 and 2022. Full or partial necropsies of 196 of the 250 stranding cases (78.4%) resulted in a diagnosis or multiple diagnoses associated with death in 126 cases. Natural disease accounted for 60.3% of stranded animals; approximately half were in poor body condition at time of death due to chronic illness. Morbillivirus and Brucella sp. infections caused mortality in eleven species, including striped dolphins and the Longman’s beaked whale. Toxoplasma infection, of anthropogenic cause in Hawaiʻi, led to the deaths of two spinner dolphins. Pygmy and dwarf sperm whales, beaked whales and pilot whales showed heavy parasitism by nematodes, cestodes and trematodes. Approximately 12.7% of stranded individuals were perinates/neonates, with three cases of dystocia with maternal mortality. Anthropogenic trauma was observed in 30.2% of strandings. In seven Cuvier’s beaked whales, cranial and/or microvascular hemorrhages were categorized as trauma. Vertebral and skull fractures were attributed to direct vessel strikes for two pygmy sperm whales, a Cuvier’s beaked whale, a humpback whale calf and a striped dolphin. Blast trauma was observed in three Fraser’s dolphins that were part of an uncommon stranding event. Significant plastic debris, and/or fishery debris were found in stomachs of six species and fatal fishhook penetration occurred in a Submitted in Support of the U.S. Navy’s 2023 Annual Marine Species Monitoring Report for the Pacific STANDARD FORM 298 Back (Rev. 8/98) bottlenose dolphin. This study highlights the value of carcass examinations in a region inhabited by small island associated populations where carcass recovery rates are low, and cetaceans face an array of natural and anthropogenic threats.

Adults of many free-ranging delphinid species cannot be reliably sexed at sea. Sexually mature, known-sex adult short-finned pilot, pygmy killer, melon-headed, and false killer whales were profiled from at-sea photos to assess proportional fin and body dimorphism. Males of all four species had larger dorsal fins proportional to anterior body length. False killer whales showed no further dimorphism, while melon-headed whales showed subtle dimorphism in dorsal fin overhang. Pygmy killer whales showed subtle dimorphism in lateral positioning of the top-most point and overhang. Lastly, short-finned pilot whales showed strong dimorphism in the leading edge, lateral positioning of the top-most point, and height of the overhang. Generalized linear models showed strong predictive accuracy for pilot and false killer whales, and moderate accuracy for pygmy killer and melon-headed whales. Results indicate that adult short-finned pilot and false killer whales can be accurately sexed in the field or via morphometrics. Adult pygmy killer and melon-headed whales have subtle sexual dimorphism that can be detected using morphometrics. The presence of dimorphism gives insight into potential reproductive strategies and social structure. Further analysis is needed to explore morphometric differences between inshore and pelagic populations of these species, as well as ontogenetic growth between life stages.

The primary goals of Winter HICEAS 2020 were to collect data required to estimate the abundance and distribution, examine the population structure, and understand the habitat of cetaceans around the main Hawaiian Islands during the winter months (January–March). There were 5 major research components to the project: visual observations for cetaceans following a line-transect survey design; passive acoustic monitoring for cetaceans using towed hydrophone arrays, sonobuoys, and autonomous drifting acoustic recorders; collection of photographs and tissue samples and deployment of satellite tags for select cetacean groups; visual observations for seabirds following a strip-transect survey design; and ecosystem measurements for assessment of cetacean and seabird habitat.